extrachloroplastic phosphoenolpyruvate carboxylase. The stimulation of pyruvate-induced C02 fixation by ox- aloacetate and 3-phosphoglycerate is thought to ...
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What is protoplast used for?
How to extract protoplasts?
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What does protoplast do in cells?
Protoplasts (106 cells ml− 1) are incubated with 10 μg TEG-coated FITC-labeled MSN (TEG-FITC-MSN) in media overnight. To remove physically adsorbed TEG-FITC- ...
28 июл. 2022 г. · In the present study, a cotton callus protoplast extraction method was tested to optimize the ratio of enzymes (cellulase, pectinase ...
Method for isolating large numbers of metabolically competent protoplasts from leaves of monocotyledons. (grasses), dicotyledons (such as spinach and sunflower) ...
29 окт. 2018 г. · The plasmid was extracted using the E.Z.N.A.® Plasmid Maxi prep kit (Omega Bio-Tek, Inc., Nor-Cross, GA, USA) following the manufacturer's ...
Protoplast extracts evolved approximately 6 micromoles of O2 per milligram of chlorophyll before photosynthesis became largely dependent on exogenous Pi while ...
19 июн. 2022 г. · Remove majority of supernatant then resuspend protoplasts with gentle swirling (intact protoplasts will resuspend quickly whereas broken cells ...
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The Plant Protoplast Digest/Wash Solution is formulated to facilitate the rapid isolation of viable protoplasts from plant tissue. Plant cells are surrounded by ...
Protoplasts are isolated cells produced by removing the surrounding cell wall either by mechanical means or by the use of cell wall degrading enzymes. Removal ...
The protoplast yield was calculated as the total number of protoplasts released in the enzyme mixture divided by the FW of tissues used for protoplast isolation ...